2 edition of Detection of sub-lethally injured Salmonellae in foods found in the catalog.
Detection of sub-lethally injured Salmonellae in foods
Julie Ann Bird
by Portsmouth Polytechnic,School of Biological Sciences in Portsmouth
Written in English
|Statement||by Julie Ann Bird.|
1 Introduction. In industrialised parts of the world, human infection with Salmonella enterica is associated, in part, with consumption of meat from food animals .Contamination of meat at all phases of production, especially by faecal material and contents of the alimentary tract at slaughter, can spread Salmonella throughout the entire food chain.. However, detection of Salmonella in. Lactose Broth is frequently used as a pre-enrichment medium when testing foods and dairy products for Salmonella spp. In dried or processed foods, Salmonella species may be sub-lethally injured and in low numbers. The presence of other bacteria as well as components of the food sample may hinder growth and recovery of Salmonella.
Low numbers and/or sub-lethally injured Salmonella cells may be present in a test food sample. Pre-enrichment in non-selective Buffered Peptone Water allows the cells time to repair and multiply before being introduced into selective culture, improving the chances of recovering Salmonella species from the sample.. Supports high growth and recovery rate: rich in nutrients, the medium produces. The detection of E. coli in food products is challenged by the emergence of injured cells of the bacterium, following exposure to sub-lethal doses of food preservation factors including heating.
Edel W, Kampelmacher EH. Comparative studies on the isolation of "sublethally injured" salmonellae in nine European laboratories. Bull World Health Organ. ; 48 (2)– [PMC free article] Fantasia LD, Sperber WH, Deibel RH. Comparison of two procedures for detection of Salmonella in food, feed, and pharmaceutical products. Appl Microbiol. A new testing kit for Salmonella promises to give the most sensitive detection results in less than a day, its manufacturers claim. Oxoid claims its DuPont lateral flow system Salmonella test has the ability to detect one colony forming unit of Salmonella in a 25g food sample within 24 hours.. The kit has its own primary enrichment medium for testing that facilitates the recovery of sub.
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The purpose of this study was to adapt these two procedures for the simultaneous rapid recovery procedure of sub-lethally injured salmonellae and listeriae from artificially contaminated foods, and to test the procedure in naturally contaminated fresh and ready-to-eat foods.
Materials and methods Bacterial strainsCited by: The capacity to detect low levels of healthy and sub-lethally injured Salmonella enterica cells in chocolate by two alternative rapid detection methods iQ-Check TM Salmonella II real-time PCR (Bio-Rad) and VIDAS® Easy SLM (BioMérieux) was assessed and compared with ISO Chocolate, a low moisture food known to support the survival of Salmonella, was challenged as food Cited by: Detection of low numbers of healthy and sub-lethally injured Salmonella enterica in chocolate Vicky Jasson (UGent), Leen Baert (UGent) and Mieke Uyttendaele (UGent) () INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY.
p In book: Salmonella - A Dangerous Foodborne Pathogen recover sub-lethally injured cells due to heat, cold, acid, or osmotic shock (Sandel et al. ; Rapid detection of Salmonella in food. Conventional culture detection methods are time consuming and labor-intensive.
For this reason, an alternative rapid method combining real-time PCR and immunomagnetic separation (IMS) was investigated in this study to detect both healthy and heat-injured Salmonella Typhimurium on raw duck wings.
Firstly, the IMS method was optimized by determining the capture efficiency of Dynabeads ® Cited by: In book: Salmonella-A Dangerous Foodborne Pathogen, Chapter: Detection of Salmonella spp.
presence in food, Publisher: InTech, Editors: Barakat S. Mahmoud, pp To demonstrate effective control of this pathogen, food businesses analyse products for the presence of Salmonella. The current European recognised detection method for Salmonella spp.
is ISO ISO is a culture based method with four consecutive steps: Non-selective enrichment to resuscitate injured or stressed Salmonella. There was no difference in the detection time of salmonellae when incubated alone or with listeriae, but listeriae grew at a slower rate in the presence of salmonellae, resulting in a delay of detection.
Overall, the detection of salmonellae and listeriae in 25 g of the tested foods required a total of 24 h. Food Microbiology,4, Recovery of sub-lethally injured bacteria from frozen foods C. Oscroft*, S. Alcockt and J. Clayden~ Department of Microbiology, Campden Food Preservation Research Association, Chipping Campden, Gloucestershire GL55 6LD, UK Received 28 August Using a "solid-repair' method, recovery of the natural microflora in frozen chicken.
Enrichment medium may also affect the detection by decreasing the concentration of marker molecules in the cells. For example, the cultivation of sub-lethally injured Salmonella in unsuitable medium can cause denaturation of certain antigens (Hahm & Bhunia, ) and repression of virulence marker genes (Cochrane & O’Connor, ).
Author: Bird JA. Search worldwide, life-sciences literature Search. Advanced Search. E.g. "breast cancer" HER2 Smith J. The isolation and detection of Salmonella from foods can present practical difﬁculties because these bacteria may be present in low numbers and often in the presence of high numbers of competitor organisms.
Moreover, cells may also be sub-lethally injured by food processing or by intrin-sic factors associated with a particular food. For these. Food poisoning/salmonella. New search | Advanced search | Search results: Login Detection of sub-lethally injured salmonellae in foods.
Author: Bird, Julie Ann. ISNI: Awarding Body: Portsmouth Polytechnic Current Institution: University of Portsmouth Date of Award: Availability of Full Text.
Item No.: Brand: Difco Details: Recover sub-lethally injured Salmonella and Listeria from foods. Mfr. Item No.: Qty.: grams Your Cost.
detection of Salmonella in foods and food ingredients. We will focus our discussion on Cultural methods typically involve the enrichment of a portion of the food sample to recover sub-lethally injured cells due to heat, cold, acid, or osmotic shock (Sandel et al. ; The detection of sub-lethally damaged cells is of.
A direct staining technique was investigated for the detection of viable Salmonella in fresh and processed meats. The technique involved overnight enrichment in BPW, extraction of Salmonella cells onto a polycarbonate membrane, followed by detection of the pathogen using anti‐Salmonella monoclonal antibody coupled with an antibody linked‐fluorescent stain (Texas Red).
Such differences represent a substantial number of sub-lethally injured cells, e.g. at 10 h the counts were 5 × 10 2 CFU ml-1 for V.
cholerae under aerobic conditions and × 10 4 CFU ml-1 under ROS-n conditions, with equivalent counts for V. cholerae O1 of 5. Optimal enrichment is important, as it must enable sub-lethally injured salmonella cells to recover and grow but simultaneously should inhibit the growth of the background flora.
The PCR protocols must be modified to prevent the problem of PCR. PREVALENCE AND IMPACT OF SALMONELLA IN PIG FECES. The detection of Salmonella in pigs is difficult, because infection does not commonly result in clinical symptoms. However, subclinical Salmonella infections in pigs are an important food safety problem because of the transmission route of Salmonella through the food chain to humans.
Salmonella in pigs can be divided in two groups. Therefore performances of rapid and reference methods for Salmonella detection in foods were compared retrospectively using data from 49 published studies.
A. Boothroyd M, Baird-Parker AC. The use of enrichment serology for Salmonella detection in human foods and animal feeds. J Appl Bacteriol. Mar; 36 (1)– Edel W, Kampelmacher EH. Comparative studies on the isolation of "sublethally injured" salmonellae in nine European laboratories.
Bull World Health Organ. ; 48 (2)–Low numbers and/or sub-lethally injured Salmonella spp. cells may be present in a test food sample. Pre-enrichment in non-selective Buffered Peptone Water allows the cells time to repair and multiply before being introduced into a selective culture, improving the chances of recovering Salmonella species from the sample.
Supports high growth and recovery rate: rich in nutrients, the medium.Rapid detection of Salmonella in foods is often limited by the high demand for the sensitivity of detection, poor physiological conditions of the target cells, and high concentration of background.